In Vitro Assessment of the Impact of Ultraviolet B Radiation on Oral Healthy and Tumor Cells

Ultraviolet radiation (UVR) is generally considered a primary tumorigenic agent. While UVR exposure has been studied, especially at the skin level, impact of UV on internal tissues and its effect appearance development tumors not yet fully examined. Although there are maximum limits for external tissues, other such as oral tissue, can be exposed to well. Over course diagnosis treatment, cells may ultraviolet radiation; however, an established limit exposure. Therefore, aim current study was examine effects ultraviolet-B (UVB) two doses (2.5 5 J/cm2) tumor (pharyngeal carcinoma tongue carcinoma) healthy (gingival fibroblasts). The viability their morphology, actin filaments, nuclei structures; expression anti-apoptotic (Bcl-2) pro-apoptotic (Bax) genes; roles caspases-3/7, 8, 9 were determined after had UVB. experiments revealed that both types cell lines showed reductions in viability, dose J/cm2. Additionally, apoptotic-like changes (rounding cells, condensation nuclei, re-organization filaments) observed all analyzed cells. genes UVB (5 induce apoptosis Moreover, analysis enhanced activity, suggesting death could caused by intrinsic extrinsic apoptosis. Accordingly, used dental practices induced nonselective apoptotic changes, thereby reducing viability.